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Core Reviews

The responses below were submitted over the years by either LSU personnel or by people from around the world. For responses to our 2021 LSU Client Survey, please download Testimonials & Comments.

Entry 34:  (2019-10-22 18:16:26).
The SACKs are super helpful! thank you so much!
– Doug Ross

Entry 33:  (2019-02-12 11:59:09).
Hi, Your information about "How to add POP-7 with a modified supply bottle?" was very interesting. Do you have any Idea about ABI 3500? I mean refilling pop-7 polymer. Best Regards. [Response, Aug. 2021: The 3500 no longer has a hard stop for when the polymer pouch would be consumed; thus, I suspect that one could pierce the pouch, add polymer, and seal the hole again... but have no 3500 instrument to test. The 3500 polymers also differ from those of the 3130; thus, I don't know if one can get the right polymer in a format that would allow for transferring polymer into an existing pouch.]
– Jamshid

Entry 32:  (2019-01-30 09:16:29).
very useful. thanks a question, when the equipment arise the message Laser power probably is too dirty? [Response: Multiple issues might generate an error message about Laser power, but they would all likely require professional service to resolve the problem.]
– erika salazar

Entry 31:  (2017-12-27 11:15:49).
This resource has been of the utmost help for me, as I was hired to be the "caretaker" of a 3130XL at a private DNA testing laboratory. I cannot even begin to stress how useful the information has been. Thank you so much!
– Anonymous

Entry 30:  (2017-07-20 10:31:22).
Good Morning, I am, Dr. Summer Siddiqui, working as a postdoctoral fellow with Dr. Binhua Ling at TNPRC. I have some samples for sequencing and want to use DNA sequencing core facility. Can you send me basic information about pricing, sample submission procedure and length of time to get results? Thanks, Summer [Response: This facility is primarily for labs at the LSU Baton Rouge campus; however, if you email us through the Contact Us link, we can discuss your potential options further.]
– Summer

Entry 29:  (2016-08-13 04:53:26).
very useful site, excellent public service!
– Cort Anderson

Entry 28:  (2016-02-18 11:41:23).
This capillary electrophoresis guide has been SO HELPFUL. Thank you!!!!!
– KP

Entry 27:  (2015-07-23 14:30:04).
Thank you for making the SACK page available. I'm learning molecular techniques for the first time, and this is by far the most helpful resource I've found!
– RH

Entry 26:  (2015-04-20 16:43:27).
Just wants to have moré información about the cost and facility payments. [Response: This facility is primarily for labs at the LSU Baton Rouge campus; however, if you email us through the Contact Us link, we can discuss your potential options further.]
– Grissel

Entry 25:  (2014-12-12 02:17:26).
Do you know how to prevent the piston rises up when you start the secuencer?. Thanks and regards. [Response: As far as I know, there is NO way to prevent the piston from initializing when the Sequencer is started. The “stop” command in the Manual Controls does not work; see "How to stop the piston?" in the 3130XL SACKS. If the sequencer goes offline (e.g., in a power failure), then one can only hope there is enough polymer in the supply bottle to keep air out of the supply tube. However, when I know that the sequencer will be turned off, I add enough polymer to the supply bottle to allow for the pump to initialize without sucking in any air.]
– Benjamin Rabanal

Entry 24:  (2014-11-26 21:39:12).
Thanks for this such an informational post. it will really help me into my projects. thanks for helping peoples like me.
– rajneesh tripathi

Entry 23:  (2014-10-17 01:59:11).
Thanks a lot for all the information. It's big help to me.
– qijun zhang

Entry 22:  (2014-09-24 09:31:00).
Great job! I really learned a lot about maitenance of our ABI3130 and to save polymer. Go on!
– Diana

Entry 21:  (2013-10-25 16:29:35).
Many thanks from Ferrara, Italy. Great job, keep it up!
– Alessandro M. Spinelli

Entry 20:  (2012-07-23 00:00:00).
Thanks so very much for your excellent site. This is all such invaluable information and collected in one place. I am very interested in Capillary washing with water to try and extend the useful life - this is fantastic, thanks again.
– Bron Matheson

Entry 19:  (2012-06-08 00:00:00).
Our lab have this machine and I have been doing the maintenance (and am almost the only user) for years. I wish I had found here earlier. This is very very very useful! Thank you! To me, whoever wrote up this protocol deserves a Nobel prize. [Response: Still no word from the Nobel committee...]
– Qinghong Yan

Entry 18:  (2012-02-07 00:00:00).
As requested, I am signing the guest book to let you know how useful I have found the 3130xl information. I haven't read all the information in full yet, but am thrilled to have found your site. The straightforward answers to questions are great, especially as I am struggling to run an instrument on my own with infrequent use - creates lots of problems! Particulary interested in the info about POP7 use and capiallary shelf life. Thank you!
– Dr Chloe Thompson

Entry 17:  (2011-12-15 00:00:00).
I wanted to thank you for compiling a basic how to run your own genomics facility. This site has been a godsend for me learning all the in's and outs of the 3130xl. Thank you so much!
– Alex Harris

Entry 16:  (2011-06-03 00:00:00).
I have 5 years experience working (STRs genotyping) with ABI 310 and ABI 3130 equipement. In your page, I have found confirmation to several issues and a lot of new ones. I am really happy since I've been looking for a place like this for ages. Thanks for all this stuff.
– Tomas Restrepo A

Entry 15:  (2011-05-09 00:00:00).
Very useful! Thanks and greetings from Poland
– Ania

Entry 13:  (2011-03-31 00:00:00).
S. Herke was very helpful with troubleshooting. He offered me a variety of suggestions for improving my results. I appreciate the time he took to explain different aspects of the reaction.
– Noelle Bryan

Entry 14:  (2011-03-31 00:00:00).
Awesome site you have.
– srikanth

Entry 12:  (2010-09-01 00:00:00).
This is a brilliant website- really useful and explains everything in a language that you can understand unlike the manuals!!
– Donna Gillies

Entry 11:  (2010-07-20 00:00:00).
This is great, thank you! Something I might be able to contribute: http://www.azcobiotech.com/reagents/NPPolymer.php inexpensive polymer, has worked very well for us. [Response: Options from other vendors can be less expensive and may work well. Do keep in mind that new spectrals are required if switching polymer vendors. Also, if your Service Agreement is through ThermoFisher (ABI), you will likely be required to put the instrument back on the standard reagents before an FSE will service the instrument.]
– evan

Entry 10:  (2010-07-17 00:00:00).
Hello, What a great site this is for a newbie like me! I have learnt so much here. Thank you very much. If you can put in training video, it will be fantastic. [Response: Unfortunately, time to make training videos is a luxury I do not have.]
– Loy Boon Pheng

Entry 9:  (2010-06-29 00:00:00).
This site has been extremely helpful in helping me to operate our 3130xl more efficiently and effectively. Thanks for the valuable info!
– Akia White

Entry 8:  (2009-12-23 00:00:00).
I have only had a quick look, but looks like a very informative site. I'll be back for a more detailed look in the near future. Well done!
– Tony Pye

Entry 7:  (2009-12-17 00:00:00).
Thank you for the userful information. Mark and I were grad students together a long time ago. shane
– Shane Sarver

Entry 6:  (2009-09-22 00:00:00).
Thanks for all of your help and hard work. This website provides useful information and resources. I especially appreciate the user-friendly submission process.
– Deb T.

Entry 5:  (2009-08-17 00:00:00).
The ABI 3130XL SACK's page is excellent and will be an indispensable resource as I learn to use our new 3130xl. Many thanks! Roger D. Bull DNA Lab Coordinator / Coordonnateur, Laboratoire d’ADN Canadian Museum of Nature / Musée canadien de la nature
– Roger D. Bull

Entry 4:  (2009-06-24 00:00:00).
Very helpful site. I'm just becoming familiar with this instrument, and the info here will really increase my cost efficiency in using it. Thanks!!
– Tim Evans

Entry 3:  (2008-07-31 00:00:00).
Much useful information, many thanks.
– Martin Whittle

Entry 2:  (2008-02-12 00:00:00).
I suggest that the info on the timeline of obtaining a user ID be placed in a non- secure area....like maybe placed on the page where we initially request a user ID ! Also , LOVE the site...valuable , user-friendly information is provided . Impressive and greatly appreciated. Kudos! [Response: Good suggestion... done.]
– Pat Arbour-Reily

Entry 1:  (2008-01-16 00:00:00).
When emailing your review, you may request that it be posted either under your real name or as by 'Anonymous'. Typically, if requested, written responses are generated within 5 business days.
– Genomics Facility