Services ... LSU Genomics Core
• Reservation-Required instruments
• Scientific Assistance
• Special Requests
• Local "Store" (buy | borrow | freebies)
• RNA & ssDNA purification
• dsDNA purification
• dsDNA Size-selection
• QC & Quantitation methods
• Next Generation Sequencing (NGS)
• DNA Sequencing or Fragment Analysis
Reservation-Required instruments
All Reservation-Required instruments
Client PC Workstation (genomics software)
Smart Check (pipette verification & training)
QuantStudio·6 (qPCR)
Veriti thermal cycler (standard PCR)
Qubit 4.0 (fluorometer)
Diagenode Bioruptor NGS (DNA shearing)
SPD1010 SpeedVac (sample reduction)
Typhoon 8600 Variable Mode Imager (phosphor screens)
Eppendorf Centrifuge 5810R (for 96-well plates)
Victor·3 Multilabel Counter (fluorescence, luminescence, visible)
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Scientific Assistance
Consultations: troubleshooting & project development.
Training: molecular biology techniques & analyses.
Information: selected GC-website resources.
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Special Requests
Reagents & Supplies: for unlisted supplies & reagents.
Services: for unlisted services
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Your Local "Store"
Reagents & Supplies: in-stock items.
BioSci Thrift Store: free or discounted items; trades; housing.
BioSci Tool Loans: temporary borrowing privileges. ↵ Back to Top
RNA & ssDNA purification
ProNex magnetic beads: (Promega – better than AMPure XP)
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dsDNA purification
GC-Precipitation (standard EtOH-EDTA)
GC-Precipitation (optimized for residual primer removal)
ExoSAP-IT Express: (enzymatic primer [ssDNA] degradation)
Columns (Zymo: DNA Clean & Concentrator-5)
ProNex magnetic beads: (Promega – better than AMPure XP)
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dsDNA Size-Selection
E-gel: 50-bp windows.
BluePippin: specific size ranges.
Select-a-Size columns (≥50, 100, 150, 200, or 300-bp)
ProNex magnetic beads: (Promega – better than AMPure XP)
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QC & Quantitation methods
Agilent Bioanalyzer: quantitation & sizing of DNA, RNA & Protein.
Qubit 4.0: quantitation of DNA, RNA, & Protein; RNA integrity.
Illumina: QC for Illumina-based NGS libraries.
SmartCheck: QC pipettes, accuracy & precision [in progress]
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Next Generation Sequencing (NGS)
Overview – NGS Services
NGS Service Providers
In-House NGS (Ion Torrent S5)
In-House Services for NGS Libraries
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DNA Sanger-Sequencing & Fragment Analysis
Overview – ABI 3130xl services
DNA Sanger-Sequencing: Service Levels
DNA Fragment Analysis: Service Levels
Sample submission & Turnaround times
Data Analysis
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