Services ... LSU Genomics Core
• Reservation-Required instruments
• Scientific Assistance
• Special Requests
• Local "Store" (buy | borrow | freebies)
• RNA & ssDNA purification
• dsDNA purification
• dsDNA Size-selection
• QC & Quantitation methods
• Next Generation Sequencing (NGS)
• DNA Sequencing or Fragment Analysis
Reservation-Required instruments
All Reservation-Required instruments
Client PC Workstation (genomics software)
Smart Check (pipette verification & training)
ABI ViiA·7 and QuantStudio·6 (qPCR)
Veriti thermal cycler (standard PCR)
Qubit 4.0 (fluorometer)
Diagenode Bioruptor NGS (DNA shearing)
SPD1010 SpeedVac (sample reduction)
Typhoon 8600 Variable Mode Imager(phosphor screens)
Eppendorf Centrifuge 5810R (for 96-well plates)
Victor·3 Multilabel Counter (fluorescence, luminescence, visible)
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Scientific Assistance
Consultations: troubleshooting & project development.
Training: molecular biology techniques & analyses.
Information: selected GC-website resources.
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Special Requests
Reagents & Supplies: for unlisted supplies & reagents.
Services: for unlisted services
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Your Local "Store"
Reagents & Supplies: in-stock items.
BioSci Thrift Store: free or discounted items; trades; housing.
BioSci Tool Loans: temporary borrowing privileges. ↵ Back to Top
RNA & ssDNA purification
ProNex magnetic beads: (Promega – better than AMPure XP)
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dsDNA purification
GC-Precipitation (standard EtOH-EDTA)
GC-Precipitation (optimized for residual primer removal)
ExoSAP-IT Express: (enzymatic primer [ssDNA] degradation)
Columns (Zymo: DNA Clean & Concentrator-5)
ProNex magnetic beads: (Promega – better than AMPure XP)
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dsDNA Size-Selection
E-gel: 50-bp windows.
BluePippin: specific size ranges.
Select-a-Size columns (≥50, 100, 150, 200, or 300-bp)
ProNex magnetic beads: (Promega – better than AMPure XP)
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QC & Quantitation methods
Agilent Bioanalyzer: quantitation & sizing of DNA, RNA & Protein.
Qubit 4.0: quantitation of DNA, RNA, & Protein; RNA integrity.
Illumina: QC for Illumina-based NGS libraries.
SmartCheck: QC pipettes, accuracy & precision [in progress]
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Next Generation Sequencing (NGS)
Overview – NGS Services
NGS Service Providers
In-House NGS (Ion Torrent S5)
In-House Services for NGS Libraries
Request Instrument Acquisition
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DNA Sanger-Sequencing & Fragment Analysis
Overview – ABI 3130xl services
DNA Sanger-Sequencing: Service Levels
DNA Fragment Analysis: Service Levels
Sample submission & Turnaround times
Data Analysis
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