Genomics Core
_{Services, supplies & instrumentation
for comparative genomics.}

Services – Reagents & Supplies

Note: If you need an item that is not listed below, please go to the Special Orders (via vendors) webpage.

Online Submission Request:

1. Log In to website, bringing you to 'Request Form' page.
2. Click on 'Reagents & Supplies' button.
3. Submit a completed request.
4. Pick up items in A628 (Life Sciences Annex).

Please note the following:

Particular items might be temporarily out of stock.

Items can be picked up only during normal hours.

Make an appointment if you want to ensure the Core will be open when you arrive.

Alternatively, you can request that your supply be placed in the 'After-Hours' Mini-fridge located in the hallway (by Room A649); however, you then assume all risk associated with an unmonitored transfer of your supply.

Discounted items relative to regular purchase pricing: SQD = Special Quote Discount; OSD = Old Stock Discount. Associated numbers indicate the approximate percent discount.

Items Typically Stocked

– qPCR:

PowerTrack SYBR Green (5-ml, ThermoFisher, PN-A46112).^SQD50

PowerTrack SYBR Green (1-ml, aliquot by Genomics Core from ThermoFisher PN-A46112).^SQD50

ABsolute Optical Adhesive film (50/box; AB1170)^SQD50

MicroAmp Optical Adhesive Film (25/box; PN4360954)^SQD50

MicroAmp Splash-Free Support Base.^{1, SQD30}

MicroAmp Applicator (Adhesive Film, PN4333183)

– Buffers/Water:

EDTA (70-150 mM)²

Resuspension-Elution buffer (TVLE, 50 ml) ³

Water (500 ml, nuclease-free, Cytivia SH30538.02)

Water (50 ml aliquot, nuclease-free)

The RNA Storage Solution (PN-AM7001; 5-ml aliquot)

– Sanger-Sequencing reagents:

BigDyeTerminator v3.1 (~800 ul)^{4, SQD30}

BigDyeTerminator v3.1 (~100 ul)^{4, SQD30}

5X Sequencing buffer⁶

Hi-Di formamide (25 ml or ~1.5 ml)⁷

Pos-Ctrl Seq. template (pGEM, 25 ng/µl)⁸

Pos-Ctrl Seq. primer (M13-Rev, 5 µM)

Pos-Ctrl Seq. primer (M13-For, 5 µM)

Primer (T7 Promoter; 20 ul @ 16.2 uM)

Primer (T3; 20 ul @ 16.2 uM)

– Tubes/Plates/Seals:

25-ml Eppendorf tubes, screw cap (PN 0030122410)⁹

Qubit 0.5-ml tubes, 100 (~34.6g; Axygen, PCR-05-C)

MicroAmp 8-Tube strips (125/box; PN-N8010580).^SQD50

MicroAmp Caps, 8 caps/strip (full/partial box; PN-N801-0535).^SQD50

MicroAmp Cap Installing Tool (PN4330015)

96-well ABI optical PCR plates (10/pack)^OSD65

96-well ABI optical PCR plates (20/pack, barcoded)^OSD30

96-well VWR PCR plates (10/pack)

96-well non-skirted PCR plate (VWR82006-636, single)

Adhesive Sealing Films (Non-Optical; 100-SEAL-PLT).^{10, OSD50}

Adhesive ThinSeal Films (Non-Optical; 100-THER-PLT).^{11, OSD50}

Adhesive MicroAmp Clear Films (Non-Optical; 20-pack).^{12, OSD30}

– Miscellaneous:

Kimwipes, ~11X21 cm, 280 wipes/box^OSD50

Kimwipes, ~38X43 cm, 140 wipes/box^OSD50

Weigh Dish (500 hexagonal dishes, 2.5"-2", Fisher Sci)^OSD50

Weighing Paper (500 sheets, 4"X4", Fisher Sci)^OSD50

N95 Respirator (H910 Plus series, Head-Loops)

Special Orders

BioSci Thrift Store items

Notes:

1. MicroAmp Splash-Free Support Base:  These are required for all work involving qPCR; otherwise, your plate can pick up fluorescent contamination, possibly even contaminating the block of the ABI qPCR instrument.
2. EDTA:  Used in the Genomics Core's EtOH protocol to clean 10-µl BigDye reactions.
3. TVLE:   a better alternative to 'water' for resuspending or eluting DNA templates. Unlike pure water, T_risV_eryL_owE_DTA (10 mM Tris; 0.05 mM EDTA; nuclease-free water) prevents extreme pH changes and mild DNase activity; however, the levels of Tris and EDTA are too low to adversely affect downstream applications.
4. BigDye (~800 ul):  stock ABI tube, ~1,600 rxn's @ 1/2 µl/rxn. Stable at -20^oC for at least 1-2 years if not thawed repeatedly; upon first thaw, vortex vigorously (~20 s) and aliquot into volumes that will be consumed within 5-10 freeze-thaw cycles.
5. BigDye (~100 ul):   aliquoted from stock tube, ~200 rxn's @ 1/2 µl/rxn. These aliquots are provided as a courtesy to labs doing minimal sequencing (e.g., <30-40 rxn's/month).
6. Sequencing buffer:  Formula is 400 mM Tris (pH 9), 10 mM MgCl₂. Provided either as the Genomics Core's "Homebrew" (25 ml, made with commercial nuclease-free liquid products) or as ABI's commercial product (1.0 ml aliquots).
7. Hi-Di Formamide:  ABI's version is highly recommended over other vendors, given that formamide from elsewhere might not be suitable for resuspending sequenced DNA. Aliquots are available for labs that perform limited amounts of sequencing.
8. Positive-control reagents:  including some positive-controls in your DNA sequencing can be tremendously helpful in trouble-shooting any weak or failed reactions; if you don't have suitable templates available, pGEM and M13 primers can be substituted.
9. Eppendorf 25-ml tubes (screw cap): The only difference between these tubes and the version labeled as "nuclease-free" is that Eppendorf actually performed a test of a random sample of tubes from Lots that were subsequently labeled as "nuclease-free" — otherwise, both versions were produced in the same clean-room facilities and can be considered "nuclease-free".
10. Adhesive Films (100-SEAL-PLT): These seals are not suitable for qPCR experiments; intended for sealing microplates. SealPlate sealing films minimize evaporation, prevent spillage and contamination between wells, and provide a secure seal. Box of 100 seals.
11. Adhesive ThinSeal Films (100-THER-PLT): These seals are not suitable for qPCR experiments; intended for sealing microplates and PCR-applications. SealPlate ThinSeal films minimize evaporation, prevent spillage and contamination between wells, and provide a secure seal. Box of 100 seals.
12. Adhesive MicroAmp Clear Films (PN4306311; ThermoFisher): These seals are not suitable for qPCR experiments; intended for sealing 96-well and 384-well PCR plates. Bag of 20 seals.

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