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Services – DNA Columns

• DNA Purification or Sample Reduction

• Online Submission Request

• Physical Submission of Samples

DNA Purification or Sample Reduction

The Core stocks DNA clean-up columns by Zymo Research. Predominantly, we use one particular kit for DNA cleanup or sample reduction, but other Zymo kits designed for specific applications can be ordered. For product specifications of all available kits, visit either the distributor's website (Genesee Scientific) or the manufacturer's website (Zymo Research).

DNA Clean & Concentrator-5 Kit — (Cat #: 11-303):
  • Clean and concentrate up to 5 µg DNA in as little as 6-µl elution with 0-µl wash residue carryover.
  • Eluted DNA is well suited for use such as PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, and arrays.
  • DNA Size Limits: 50 bp to 23 kb.

  • Other in-stock Zymo Kits (expired, but likely still effective):
  • Quick-gDNA MicroPrep (Cat. #D3020)
  • Quick-DNA Universal Kit (Cat. #D4068)
  • ZR Genomic DNA-Tissue MicroPrep (Cat. #D3040)
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    Online Submission Request

  • Log In to the website, and click on "Services Page":
    • Make the necessary 'selections'.
    • In the "Client Memo" box, specify the desired elution volume and any other information. [Note: Unless otherwise requested, DNA will be eluted with TVLE (10 mM Tris, 0.05 mM EDTA).
    • Click the 'check box', and submit your request.
  • If you make an error in your request, you may revise that request if you do so before we begin processing it.
  • You will receive an automated email when your samples are processed and ready for pick-up.
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    Physical Submission of Samples

  • Tubes:  Samples exceeding ~25 ul should be submitted in 0.5-1.5 ml tubes (standard or Eppendorf LoBind); otherwise, 0.2-ml tubes are acceptable.
  • Label Submission:  Upon successful submission of your online request, the 'Submission #" will appear in your 'Submission History'. Then, label the tube rack itself with:
    • Submission #;
    • Date;
    • PI's Last Name; and,
    • Submitter name or initials.
  • Delivery site:  Transfer tubes to a Genomics Core rack (taking your own rack back to your lab), and put samples in the Mini-fridge (by sink) in the Genomics Core (A628 Life Science Annex).

    [Note: If the Core is locked, put your samples in the plastic "After-Hours" GC drop-box located in the adjacent Cold Room (beside sink, Rm. A650).]

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