Services
– ProNex Magnetic Beads
ProNex Size-Selective Purification System
The ProNextm Size-Selective Purification System enables rapid and efficient magnetic resin-based purification of dsDNA for NGS, PCR and general molecular biology applications. Based on Promega's comparison data to AMPuretm XP beads, the novel reagent formulation of ProNex beads provides significantly improved selectivity, reproducibility and yield.
ProNex beads were developed to remove gDNA from "short-read" NGS libraries, and technical support states that the product removes >99% of intact genomic DNA at initial levels up to 200 ng/µl. By varying the volume ratio of "ProNex
Genomic DNA: ProNex beads can also be used to purify genomic DNA. For instance, Promega's Oxford Nanopore Sequencing application note (vendor pdf) outlines the procedure in which the goal was the retention of high-quality intact genomic DNA. Essentially, one follows the basic "single-selection" protocol, using whatever ratio will retain the smallest desired fragments from the genomic DNA.
When testing ProNex, the Genomics Core found that certain protocol modifications or clarifications improved results (LSU_ProNex_Dual-Size-Select_100-700bp.docx). In particular, the standard protocol left enough surface tension in the sample to cause tiny amounts of the magnetic beads to detach from the main bead mass on the magnet, resulting in residual beads in the cleaned samples. This problem was eliminated by first bringing samples to a final concentration of ~0.005% Tween-20, which broke the surface tension of the sample.
Online Submission Request
- Make the necessary 'selections'.
- In the "Client Memo" field, specify your size-selection parameters.
- Click the 'check box', and submit your request.
Physical Submission of Samples
- Submission #;
- Date;
- PI's Last Name; and,
- Submitter name or initials.