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Scott Herke

Scott Herke As manager of the Genomics Core for the College of Science, I provide DNA sequencing services, access to molecular biology equipment and technique trouble-shooting advice. My experience in academic biology includes molecular biology, population genetics, embryology, and fisheries field research. Beyond academia, I served as a project manager for five years in an environmental-regulatory program (New England Division, U.S. Army Corps of Engineers). My strengths include extensive experience in troubleshooting techniques, a broad background in molecular and field research techniques, and a dedication to designing better ways to achieve work goals.
My Molecular Toolbox
Education
Selected Publications

My Molecular Toolbox:

  • DNA sequencing
       – Sanger-Sequencing.
       – NGS (Ion Torrent S5; Ion Torrent PGM; Illumina GAIIx;)
  • Cloning (PCR products)
  • PCR and qPCR, including primer design
  • PCR-RFLP and allozyme genetic analysis
  • Protein & Nucleic Acid Electrophoresis
       – BluePippin (Sage Science)
       – E-gels (ThermoFisher)
       – starch gels
       – agarose gels
       – polyacrylamide gels
  • DNA and RNA isolation and purification
  • DNA and RNA quantification
       – Qubit assays
       – Agilent Bioanalyzer
       – Nanodrop
  • RNAi (gene inhibition) and RNA transcription
  • Cell culture
       – flasks
       – OptiCell cassettes
       – Cytodex-1 microcarrier beads

    • Education:

  • Ph.D. Biological Sciences 1999, Louisiana State University
  • M.S. Zoology 1988, University of Maine at Orono
  • B.S. Forestry 1986, Louisiana State University

    • Selected Publications:

  • Wang, K., F. Donnarumma, S.W. Herke, C. Dong, P.F. Herke, K.K. Murray. 2019. RNA sampling from tissue sections using infrared laser ablation. Analytica Chimica Acta 1063: 91-98.
  • Wang, K., F. Donnarumma, S.W. Herke, P.F. Herke, K.K. Murray. 2017. Infrared Laser Ablation Sample Transfer of Tissue DNA for Genomic Analysis. Analytical and Bioanalytical Chemistry 409(17): 4119-4226. doi:10.1007/s00216-017-0373-z (July, Paper in Forefront).
  • Bakshi, A., S.W. Herke, M.A. Batzer and J. Kim. 2016. DNA methylation variation of human-specific Alu repeats. Epigenetics 11(2):163-173 (DOI:10.1080/15592294.2015.1130518).
  • McLain, A.T., T.J. Meyer, C. Faulk, S.W. Herke, J.M. Oldenburg, M.G. Bourgeois, C.F. Abshire, C. Roos, and M.A. Batzer. 2012. An Alu-Based Phylogeny of Lemurs (Infraorder: Lemuriformes). PLoS ONE 7(8):e44035. doi:10.1371/journal.pone.0044035.
  • Herke, S.W., J. Xing, D.A. Ray, J. Zimmerman, R. Cordaux, and M.A. Batzer. 2006. A SINE-based dichotomous key for primate identification. Gene 390:39-51.
  • Cordaux R., D.J. Hedges, S.W. Herke and M.A. Batzer. 2006. Estimating the retrotransposition rate of human Alu elements. Gene 373:134-137.
  • Callinan†, P.A., J. Wang†, S.W. Herke†, R.K. Garber, P. Liang, and M.A. Batzer († equal contributor). 2005. Alu retrotransposition- mediated deletion. Journal of Molecular Biology 348:791-800.
  • Herke, S.W., N.V. Serio and B.T. Rogers. 2005. Functional analyses of tiptop and Antennapedia in the embryonic development of Oncopeltus fasciatus suggests an evolutionary pathway from ground state to insect legs. Development 132:27-34.
  • Garber, R.K., D.J. Hedges, S.W. Herke, N.W. Hazard and M.A Batzer. 2005. The Alu Yc1 Subfamily: Sorting the Wheat from the Chaff. Cytogenetic and Genome Research 110:537-542.
  • Herke, S.W. and D.W. Foltz. 2002. Phylogeography of two squid (Loligo pealei and L. plei) in the Gulf of Mexico and northwestern Atlantic Ocean. Marine Biology 140:103-115.

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