Suspension of Self-Service
• Suspension Notice
• General Considerations
• qPCR Samples
• Other Sample Types
• Victor·3 Plate Reader
Covid-19 Policy (Fall 2022): Clients are encouraged to wear a mask if planning to be in the facility for more than a few minutes. A mask is not needed if you are simply depositing your samples in the A628 Mini-fridge (by sink).
Exceptions?: If the protocols noted below are not sufficient for your needs, use the "Contact Us" link to request an exception.
Suspension Notice
– Revised 16 March 2022.
Due to a lack of Service Agreements to cover instrument repairs,
only facility staff may operate any of the instruments in the facility.
Thus, all samples for Biosci-Booked (Genomics) requests will be processed by staff as described below.
This policy will be revisited when, and if, Service Agreements are re-instated on facility instrumentation.
General Considerations
Samples will be processed by Scott Herke.
Reservations are required; if you do not have access to Biosci-Booked (Genomics), please see notes at right regarding how to acquire registration.
Samples might be processed later than specified in the reservation; if this is an issue for you, please consult with Scott ahead of time.
For time-sensitive samples, ensure that Scott will be able to process them within the required time-frame before you prepare the samples for submittal.
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qPCR Samples
– Revised 16 March 2022.
Rules & Cautions (details at ABI ViiA·7 & QuantStudio 6):
– Choice of qPCR plates and seals;
– Cradle-to-Grave plate protection;
– Cold-Reaction Setup; and,
– Applying Optical Adhesive Seals.
Create or analyze qPCR run files: Download
v1.3 of QuantStudio-6/ViiA7 qPCR freeware [Warning: v1.7 is not
compatible with our qPCR platforms].
Biosci-Booked (Genomics): Reserve a time-slot sufficient to complete your assay; if total run time is unknown, reserve a 2-hr block.
Run File: Email your ViiA7 or QuantStudio-6 Run file (*.eds) to the Genomics Facility. [Note: Do not email the file to Scott's LSU account; your file is likely to become 'buried' and 'lost' if you do!]
Plate Identification: Write your
Name
& Platform
(ViiA7 or QS6):
– directly on the Plate itself (not just the "covering"); and,
– at the end of your File Name (*.eds).
Plate Submittal: your samples...
– must be put
in the Mini-fridge next to sink in Genomics Facility (A628); if concerned about potential photo-bleaching through the glass door, wrap your samples in foil.
– must be submitted either in a Splash-Free qPCR rack or wrapped in a clean Kimwipe (& wrapped in foil or inserted into a ZipLock bag).
– should be vortexed before submittal to eliminate optical anomalies from inadequate mixing (as recommended by our qPCR Field Application Specialist at ThermoFisher).
Facility Protocol: immediately prior to the run, qPCR plates are:
– centrifuged (2500 rcf, 1 min); and,
– handled in accordance with instructions included with the plate.
Data/files will be returned by email.
Plate Retrieval: Completed plates/racks will be placed in the Cold Room (A650) next to the facility; after you receive your data, please retrieve your plates/racks as soon as possible.
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Other Sample types
– Revised 16 March 2022.
For the appropriate instrument, reserve a time-slot sufficient to complete your assay.
Label samples in accordance with the specific Service requested. At a minimum, the identity of each sample should be unambiguous and the plate or rack should be tagged with the Submission Number and your Name; in general, instructions for labeling DNA sequencing samples (see How to submit samples to Genomics Facility?) will be applicable to most service requests.
Put samples in the Mini-fridge (by sink) in the Genomics Facility (A628 Life Science Annex). For tubes, transfer them to a Genomics Facility rack, taking your own rack back to your lab – do
not leave your own re-usable rack or box.
Sample Information: Provide whatever information is needed to process your samples. Either a note with the samples or an email (Genomics Facility) is suitable.
Data/files will be returned by email; when necessary, samples will be returned through the same mini-fridge noted above.
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Victor·3 Plate reader
– Revised 16 March 2022.
For access to the Victor·3, please use the "Contact Us" link above to submit a brief description of your research needs (e.g., assay time required, frequency, & "hands-on" vs. "programmable" process).
If it is not practical to hand over your samples for "Victor·3" processing, you will be allowed to process your own samples – provided you agree to wear a mask and to avoid handling anything else in the facility.
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