Documents for Equipment & Protocols
Note: The pdf documents below are original vendor documents and were not designed for use with screen reader applications.
Accuris Series Tx (analytical balance – pipette verification)
- Entry in progress (vendor pdf)
- Science Aid Center
- Real-time PCR Handbook 2.0 (2024; periodically updated)
- TaqMan Gene Expression, User Guide (vendor pdf)
- MIQE: towards reproducible qPCR (journal pdf, 2017)
- Case study using MIQE guidelines (vendor pdf, 2011)
- qPCR Tutorial Videos (YouTube: BioRad, 2019)
- Primer Express v3.0, User Guide (vendor pdf)
- Protein Thermal Shift (PTS) (vendor pdf, 2022)
- Protein Thermal Shift (PTS) (online user guide)
- Manual for QuantStudio·6 (vendor pdf)
- QuantStudio-6 qPCR, freeware,
v1.3 only (PC)
– Warning: v1.7 isnot compatible with our platforms.
- ABI Veriti PCR Manual (vendor pdf)
- Science Aid Center
- Sequencing Secrets Revealed.docx
- General Sequencing Instructions.docx
- Full-Service DNA Sequencing Instructions (GC webpage)
- Templates EtOH-Precipitation (Plate protocol).docx
- Templates EtOH-Precipitation (Tube protocol).docx
- Sequencing EtOH-Precipitation (Plate protocol).docx
- Sequencing EtOH-Precipitation (Tube protocol).docx
- ABI Standard Dye Sets.docx
- DNA Sequencing by CE Chemistry Guide (vendor pdf)
- DNA Fragment Analysis by CE User Guide (vendor pdf)
- DNA Sequencing Setup & Troubleshooting (vendor pdf)
- NanoDrop tips (re: 260/230 & 260/280 ratios) (vendor pdf)
- Bioruptor-NGS User-Manual-v1.5 (vendor pdf)
- Bioruptor NGS DNA-Shearing protocol (vendor pdf)
- Bioruptor NGS DNA-Shearing (Small-vol) (vendor pdf)
- Bioruptor Chromatin Shearing Guide (vendor pdf)
- Bioruptor NGS RNA-Shearing protocol (vendor pdf)
- in progress
- The RIN: an RNA integrity number for assigning integrity values to RNA measurements
(BMC article [pdf] which explains the RIN concept and shows a graphic of the different regions that make up the RIN estimate for the Bioanalyzer assay.) - Interpreting mRNA electropherograms (vendor pdf)
- Mastering the Bioanalyzer DNA-HS assay.docx
- Agilent Bioanalyzer Troubleshooting Guide (vendor pdf)
- E-gel Technical Guide (vendor pdf)
- Tips for E-gels.docx
- BluePippin User Manual (vendor pdf)
- SpeedVac SPD1010 User Manual (vendor pdf)
- SpeedVac Rotor-&-Tube Selection Guide (vendor pdf)
- 5810R Quick Guide.docx
- 5810R User Manual (vendor pdf)
- Typhoon Quick Guide.docx
- Typhoon User Manual (vendor pdf)
- Typhoon Application Note 1 (vendor pdf)
- Typhoon Application Note 2 (vendor pdf)
- Principles-Methods: Fluorescence Imaging (vendor pdf)
The Genomics Core does not have a Nanodrop; however, next door to this Core (in A624), there is a Thermo NanoDrop 2000 (micro-volume spectrophotometer) for use by members of the Department of Biological Sciences. Visit Common Room for a partial listing of additional equipment and restrictions upon its use.
- Nanodrop Nucleic Acid Guide (vendor pdf)
- Nanodrop Protein Guide (vendor pdf)
- Qubit 4.0 Fluorometer User Manual (vendor pdf)
- Qubit 4.0 Fluorometer Quick-Guide (vendor pdf)